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Fig. 3 | BMC Biology

Fig. 3

From: Experimental evidence for enzymatic cell wall dissolution in a microbial protoplast feeder (Orciraptor agilis, Viridiraptoridae)

Fig. 3

Free Orciraptor flagellates stained with the anti-GH5_5 pAb and two secondary antibodies. A Weak signals of both secondary antibodies (Cy3 and DyLight® 488) co-localise in cytoplasmatic granules (arrows). Food remnants show some fluorescence as well. B Cell prepared with the same protocol but without primary pAb does not contain any granules that fluoresce in both channels. All fluorescence micrographs represent single focal planes captured by widefield microscopy. Scale bars = 5 μm

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