Fig. 2

Copper differential gene expression and tissue analysis. A Venn diagrams showing the degree of overlap between the differentially expressed genes in response to copper across the three tolerant and three sensitive strains. The numbers represented in red are up-regulated genes, those in blue down-regulated genes, and those in orange are the genes with discordant changes in expression between tolerant and sensitive strains (Table 1). Expression data obtained from mated female whole-body RNA-seq (3 biological replicates of 20 females each for treated and control conditions). B Volcano plots of gene expression in tolerant (left) and sensitive strains (right). The horizontal dashed line represents the minimum adjusted p-value threshold (0.05), while the vertical dashed lines represent the fold change thresholds (log₂(1.5) = 0.58). C Classification of the tolerant DEGs and sensitive DEGs according to the levels of expression that these genes have in the carcass, digestive system, head, and ovary according to the DGET expression database