Fig. 4From: The localization of Toll and Imd pathway and complement system components and their response to Vibrio infection in the nemertean Lineus ruberRelative expression of immune genes in response to infection to Vibrio diazotrophicus. Expression of immune genes in the different time points was normalized to the gene expression levels in the control animals. The fold change was calculated using the 2−ΔΔCT method [63], standardizing to 1 the expression level for control animals. Asterisks indicate significant differences in the gene expression between control and infected animals, being evaluated by performing statistical ANOVA tests (*p-value < 0.05; **p-value < 0.01). Bars indicate the standard error between infected biological replicatesBack to article page