Fig. 4
From: Egfr signaling promotes juvenile hormone biosynthesis in the German cockroach
Egf ligands and Egfr activate Ras/Raf/ERK signaling pathway in the CA. A–B” Western blotting analysis of phospho-AKT, AKT, phospho-CaMKII, phospho-ERK, ERK and JHAMT in the CA after Egfr (A) or Egf RNAi (B). Quantification of the band intensity of phospho-AKT, phospho-CaMKII, phospho-ERK, and JHAMT protein levels (A’ and B’). C–E Effects of treatment with inhibitor Kobe0065 on the expression levels of Ras85D (C), Jhamt (D), and Cyp15A1 (E) in the head. F–F’ Western blotting analysis of phospho-AKT, AKT, phospho-CaMKII, phospho-ERK, ERK, and JHAMT in the CA after treatment with Kobe0065 (F). Quantification of the band intensity of phospho-AKT, phospho-CaMKII, phospho-ERK, and JHAMT protein levels (F’). *P < 0.05, **P < 0.01, ***P < 0.001, ns (not significant, P > 0.05), n = 3