Fig. 5

Ras/Raf/ERK downstream transcription factor Pnt promotes JH biosynthesis. A–B Effects of Pnt RNAi on the expression levels of Jhamt and Cyp15A1 in the CA (A–A”) and Kr-h1 in the fat body (B). C–C’ Western blotting analysis of JHAMT protein level in the head (C). Quantification of the band intensity of JHAMT protein levels (C’). *P < 0.05, **P < 0.01, n = 3. D–E Effects of Pnt RNAi on the size of maturing oocytes (D) and each follicle cell as well as follicular patency formation (E). Quantification of the length of the maturing oocyte (D’) and the area of each follicle cell (E’). ***P < 0.001, n = 19 or 20. Arrow: follicular patency, blue, DNA; red, F-actin; scale bar: 1000 μm or 20 μm. F JH III titer measurements in the hemolymph. **P < 0.01, n = 3. G–H Effects of Pnt RNAi on the morphology of CA (G) and size of CA cell (H). Blue, DNA; red, F-actin; scale bar: 300 μm or 10 μm. Quantification of the area of CA (G’) and each CA cell (H’). ***P < 0.001, n = 15 or 20