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Fig. 1 | BMC Biology

Fig. 1

From: Male and female mice display consistent lifelong ability to address potential life-threatening cues using different post-threat coping strategies

Fig. 1

Male and female mice have comparable ability to successfully address approaching sky predators. A Experimental flow chart. B Schematic showing the experimental setup: a cylindrical open field, a rectangular refuge, and an infrared touchscreen frame below. Visual looming stimuli were presented automatically in the open field. C Representative movement trajectory in the looming stimuli setup. The red dotted circle represents the center, within which looming stimuli can be triggered. The small blue circle represents a representative sample of the location where a mouse was exposed to looming stimuli. D Vaginal cytology representing each stage of the estrous cycle. Three cell types were identified: leukocytes (circle), cornified epithelial (black arrow), and nucleated epithelial (white arrow). Estrous cycle stages include proestrus, estrus, metestrus, and diestrus from left to right. EM Between-group comparisons of E escape percentage following looming stimuli, F the latency to initiate flight behavior following looming stimuli onset, G the latency to return to the refuge following looming stimuli onset, H the mean speed of return to the refuge following looming stimuli onset, I the time spent in the refuge following looming stimuli onset, J the ratio of time spent in the center during the 5-min acclimation period, K the ratio of time spent in the refuge during the 5-min acclimation period, L the mean speed during the 5-min acclimation period to the arena, and M the distance of initial location at stimuli onset to refuge following looming stimuli onset. *p < 0.05. Scale bar, 125 μm. Data are expressed as mean ± SEM

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