Fig. 2

Tissue-specific expression profile of the Atlantic cod aicda isoforms. A Constitutive expression of Atlantic cod Gm-aicda (top panel) and T-Gm-aicda (middle panel) transcripts was compared to ef1-α (bottom panel) in 19 different tissues through RT-PCR. Purified Atlantic cod macrophages, isolated from different fish individuals, were stimulated with Poly(I:C) (pIC) and used as negative controls for aicda expression (MQ-pIC). The splenic sample of pIC-challenged fish, different from the tissue panel fish, at 24 HPI was used as positive controls for Gm-aicda expression. B Gm-aicda transcripts expression during Atlantic cod embryogenesis was analyzed in a mixture of fertilized eggs and cleavage-stage embryos that were collected after communal spawning and distributed into three tanks. Samples of ~ 180 eggs/embryos per tank were collected from 12 h post-fertilization (day 0) until the yolk-sac absorption stage (day 20) and flash frozen. Five micrograms of total RNA extracted from each sample was used for cDNA synthesis, and 2 µl of diluted cDNA (corresponding to 50 ng of initial RNA) was reverse transcribed using isoform-specific Gm-aicda primers. The expression of the Atlantic cod elongation factor 1-α (ef1-α) was studied as a house-keeping gene. C Analysis of Atlantic cod aicda transcript expression upon immune stimulation. The expression of aicda gene was analyzed 6 or 24 h post injection with either pIC or with Formalin-killed typical A. salmonicida (ASAL). Gm-aicda transcript expression was normalized to rplp1 and atps expression, and the sample with the lowest normalized expression was used as calibrator. Data are represented as mean ± SEM (n = 10). Asterisks represent a significant difference between an immune-challenged group and the corresponding PBS-injected control group (*: p < 0.05; **: p < 0.01). The expression fold-change values are shown below the graphs