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Fig. 2 | BMC Biology

Fig. 2

From: Sex-specific expression of pheromones and other signals in gravid starfish

Fig. 2

Expression of potential attractants and spawning factors in COTS tissues/organs. A Expression of 84 putative attractant CDS [28]. Male and female expression profiles are grouped by tissues/organs. Heatmap of expression levels in eight tissues/organs combine all analysed CEL-Seq2 data. Depicted values illustrate scaled (z-score) expression levels based on TPM normalised reads from seven females and six males (except radial nerve, which is from five males). Pie charts depict the proportion of highly expressed genes, per tissue, that fall into broad secreted protein classes (blue, hydrolytic enzymes; light green, other enzymes; orange, enzyme inhibitors; green, structural/signalling proteins; red, conserved uncharacterized proteins; yellow, uncharacterised proteins). B Individual COTS expression profiles of the 37 putative attractants that are significantly differentially expressed between the testes and the ovaries. CDS names are listed to the right. Left heatmap, quartile analysis of the mean transcript abundance; Q4, most highly expressed CDS; Q1, lowest expressed CDS; and 0, expression was not detected. Right heatmap, TPM normalised expression. Star (*) indicates the COTS-specific ependymin-related proteins. C Heatmap of 194 putative secreted proteins upregulated in male tissues/organs, 68 of which are highly expressed in the testes. Highlighted are conserved CDS of interest (Additional file 2: Table S2.3)

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