Fig. 6

Inhibiting Wnt secretion dampened the therapeutic effects of BBR on colitis. A WB results showed activation of β-Catenin in rectal samples of colitis mice, colitis mice receiving IWP-2, colitis mice receiving BBR, and colitis mice receiving BBR and IWP-2, n=3 per group. B–E The body weights (B), colon length (C), and histological scores (E) were determined and H/E and Alcian Blue staining of rectal sections was performed (D). n=3 per group. Scale bars, 50 μm. Data are presented as means±SEM in A–C and E. Two-way ANOVA with Fisher’s LSD post hoc analysis (α = 0.05) was applied in A–C and E. p<0.05 was considered as statistically significant.