Fig. 7

BBR promotes expression of Wnt genes via circadian rhythm. A qPCR analysis of various circadian genes in stromal cells from normal mice, colitis mice, normal mice receiving BBR, and colitis mice receiving BBR, n=4 per group. B Western blot analysis revealed that the colon stromal cells of the colitis mice showed a decrease in Arntl and Clock, which was rescued by BBR. Right panels: quantitation data. n=3. C qPCR analysis of Arntl, Clock, Nr1d1 in cultured stromal cells treated by different dose of BBR, n=3 per group. D qPCR analysis of Wnt6, Wnt8b, Wnt9b,Wnt10b, and Wnt11 in cultured stromal cells treated with different dose of BBR, n=3 per group. E Expression of Arntl in cultured stromal cells treated by different concentrations of Arntl siRNA, n=3 per group. F qPCR analysis of Wnt6, Wnt8b, Wnt9b, and Wnt10b but not Wnt11 in Arntl siRNA knock-down cells (400 nmol) compared to control. n=3 per group. Data are presented as means±SEM in A–F. Unpaired two-tailed Student’s t test was applied in C–F, two-way ANOVA with Fisher’s LSD post hoc analysis (α = 0.05) was applied in A and B. p<0.05 was considered as statistically significant