Fig. 3
From: Pathogen-derived mechanical cues potentiate the spatio-temporal implementation of plant defense
Pathogen-derived cues shape the reorganization of CMTs in a stress fluctuation ring around the colonized area. A Biomechanical model of infected leaf. Healthy tissue was initially under isotropic tension (left). The infection releases progressively the internal tension (from left to right) that reshuffle internal stress through tissue and creates a pathogen-derived overstretched ring around the colony. B Infection-derived stress (Y-axis) relative to distance from the lesion (X-axis) and the size of the virtual lesion (colors). The shape of the stress-distance profiles was only dependent on the size of the lesion. For lesions below 6 mm of radius, the width of the overstretched ring was larger than 2mm. C Leaf overstretched proportion (w0/rl, Y-axis) was only dependent on the relative size of the infection (ri/rl). The right Y-axis shows the shortest delay in hours between the pathogen-derived stress fluctuation and contact with the lesion (τ) assuming elastic tissue, constant lesion growth speed, and a representative time of 24h to infect a whole A. thaliana leaf (τl). Stress fluctuations arise in the typical time of 1 h ahead of contact with the lesion. D Cumulative overstretched proportion of leaves relative to growth of the lesion. Almost 100% of cells were overstretched during the infection. E Patterns of pathogen-derived stretch and CMT orientation within a 0.5 mm (left) and 2.5 mm (right) range from the area colonized by S. sclerotiorum at 24 hpi and 26 hpi, measured from the analysis of confocal micrographs (N=42 and 94 cells respectively). Individual measures are shown as bars along the x-axis, the % of cells with CMTs oriented within 0–30°, 30–60°, and 60–90° of the pathogen-derived stretch is indicated above the graph, and slope of the linear model indicated the temporal trends of the distribution