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Table 2 SARS-CoV-2 detection in testicular parenchyma

From: High SARS-CoV-2 tropism and activation of immune cells in the testes of non-vaccinated deceased COVID-19 patients

 

Genetic evaluations

Protein evaluations

Infection of VERO cells

Patients

E gene (RD) (CT)

RNP ctrl (CT)

N1 gene (SP) (CT)

β-actin ctrl (CT)

LSPR-nanosensor

N shift (nm)

LSPR-nanosensor

S shift (nm)

IF

Spike (−/+)

1st day-

N1 gene

(CT)

2nd day-

N1 gene

(CT)

RNP

ctrl

(CT)

+

RNA

(CT)

−

RNA

(CT)

IF

Spike

(−/+)

CTRL #1

-

20

-

18.55

0 (−)

0 (−)

-

-

-

20

-

-

-

CV-19 #1

-

23

32.78 (+)

24.59

31.5 (+)

51 (+)

+

34 (+)

33 (+)

29

29 (+)

28 (+)

+

CV-19 #2

-

27

36.99 (+)

18.50

13.2 (+)

18.9 (+)

+

34 (+)

34 (+)

27

32 (+)

33 (+)

-

CV-19 #3

-

22

36.34 (+)

6.36

1.2 (−)

17.5 (+)

+

34 (+)

31 (+)

30

26 (+)

25 (+)

+

CV-19 #4

-

20

35.96 (+)

19.25

11.2 (+)

17 (+)

+

34 (+)

32 (+)

29

29 (+)

28 (+)

+

CV-19 #5

-

20

36.14 (+)

20.03

9.9 (+)

20.9 (+)

+

32 (+)

27 (+)

32

23 (+)

25 (+)

+

CV-19 #6

-

21

36.22 (+)

10.30

13.2 (+)

15 (+)

+

34 (+)

32 (+)

29

27 (+)

27 (+)

-

CV-19 #7

-

20

35.37 (+)

17.67

4.5 (−)

12.4 (+)

+

34 (+)

32 (+)

25

-

-

+

CV-19 #8

38 (+)

20

35.95 (+)

19.34

17.5 (+)

17 (+)

+

34 (+)

34 (+)

26

-

-

+

CV-19 #9

-

21

-

19.81

12.9 (+)

17.5 (+)

+

35 (+)

34 (+)

27

32 (+)

35 (+)

-

CV-19 #10

-

23

34.03 (+)

20.71

7.2 (+)

13.6 (+)

+

34 (+)

32 (+)

30

-

-

-

CV-19 #11

-

23

35.65 (+)

16.27

8.7 (+)

16.5 (+)

+

34 (+)

33 (+)

30

29 (+)

33 (+)

-

  1. CV-19 COVID-19 patient, RNP RNAse P gene, RD routinary diagnosis, SP specific primers, CT cycle threshold, LSPR localized surface plasmon resonance, IF immunofluorescence, Spike spike protein; (+), detection of viral content; (-), non-detection of viral content. Positivity in the RT-qPCR is detected when the CT is lower than 40, and in the LSPR nanosensor is when the shift is higher than 5 nm