Fig. 2

Knockdown of SMYD3 inhibits HCC carcinogenesis and invasion. a, b SMYD3 knockdown decreases the cellular proliferation of LM3 cells and SK-HEP-1 cells. EdU incorporation assays were performed using a fluorescence method. Representative images and statistical analyses are shown. c, d Colony formation assays of LM3 and SK-HEP-1 cells stably infected with SMYD3 shRNA, shSCR, Flag-SMYD3 or vector were maintained in culture media for 10 days prior to staining with crystal violet. Representative images and statistical analyses are shown. e, f LM3 and SK-HEP-1 cells stably expressing Flag-SMYD3, vector or the indicated lentivirus-delivered constructs (shSCR or shSMYD3) were subjected to growth curve analysis by counting the numbers of living cells. g, h Wound-healing assays of SMYD3-depleted LM3 and SK-HEP-1 cells were performed. Representative images and statistical analyses are shown, *P < 0.05, **P < 0.01 (two-tailed t-test). i Transwell invasion assays of LM3 and SK-HEP-1 cells were performed following transfection with the indicated specific siRNA. The invaded cells were stained and counted. The images represent one field under microscopy in each group, and statistical analyses are shown. **P < 0.01 (two-tailed t-test). j TUNEL assays of LM3 cells following transfection with the indicated specific siRNA were performed. The apoptotic cells were stained and quantified. The images represent one field under microscopy in each group, and statistical analyses are shown. **P < 0.01 (two-tailed t-test). k The interference or overexpression efficiency of SMYD3 in LM3 and SK-HEP-1 cells is shown