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Fig. 7 | BMC Biology

Fig. 7

From: SMYD3 associates with the NuRD (MTA1/2) complex to regulate transcription and promote proliferation and invasiveness in hepatocellular carcinoma cells

Fig. 7

Identification the different target genes between SMYD3/NuRD (MTA1) complex and SMYD3/NuRD (MTA2) complex. a A heatmap presents the selected target genes that were coregulated by SMYD3 and MTA1 (or MTA2) in the RNA-seq analysis. b LM3 cells were infected with control siRNA, SMYD3 siRNA, MTA1 siRNA, or MTA2 siRNA. RT–PCR analyses of partial transcriptional target expression in LM3 cells infected with the indicated siRNAs are shown. c qChIP analysis of the selected promoters was performed using antibodies against IgG, SMYD3, MTA1, and MTA2. Results are represented as the fold change over IgG with glyceraldehyde 3-phosphate dehydrogenase as a negative control. d The chromatin-bound proteins in Control siRNA and SMYD3 siRNA transfected LM3 cells were extracted and subjected to analysis to determine the indicated protein levels. Total histone H4 served as a loading control. e, f KEGG pathways enrichment analysis and GO analysis of the target genes exclusively regulated by SMYD3

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