Fig. 8

Inhibitors of SMYD3 effectively inhibit the proliferation of HCC cells. a MTA1, MTA2 knockdown decrease the cellular proliferation of LM3 cells. EdU incorporation assays were performed using a fluorescence method. Representative images and statistical analyses are shown. b Transwell invasion assays of LM3 cells were performed following transfection with the indicated specific siRNA. The invaded cells were stained and counted. The images represent one field under microscopy in each group, and statistical analyses are shown. **P < 0.01 (two-tailed t-test). c LM3 cells transfected with the indicated plasmid or/and the indicated lentivirus-delivered constructs (shSCR or shSMYD3) were subjected to growth curve analysis by counting the numbers of living cells. d Transwell invasion assays of LM3 cells were performed following transfection with the indicated lentivirus-delivered constructs (shSCR or shSMYD3) or/and the indicated plasmid. The invaded cells were stained and counted. The images represent one field under microscopy in each group, and statistical analyses are shown. **P < 0.01 (two-tailed t-test). e, f MTT assays were performed to analyze the viability of LM3 cells treated with an inhibitor of SMYD3 (EPZ031686 or BCI121) at different times and concentrations. g, h Colony formation assays were performed to analyze the cell proliferation ability of LM3 and SK-HEP-1 cells treated with EPZ031686 or BCI121