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Fig. 4 | BMC Biology

Fig. 4

From: CD4+ and CD8+ regulatory T cell characterization in the rat using a unique transgenic Foxp3-EGFP model

Fig. 4

Suppressive activity of TCR+CD4+EGFP+ and TCR+CD8+EGFP+ cells in an MLR. An MLR was performed by co-culturing in a 1:1 ratio spleen CD4+CD25 Tconv cells from SD/Crl rats (MHC haplotype u) with enriched spleen APCs from Lewis 1A (MHC haplotype a) rats. Spleen Tregs from wild-type (WT) or Foxp3-EGFP SD/Crl rats were sorted based on their surface phenotype using previously defined markers. For CD4+ Tregs, TCR+CD4+CD25+CD127low/− and for CD8+ Tregs, TCR+CD8+CD45RClow/−. CD4+ and CD8+ Tregs were then sorted based on the expression or not of EGFP. The four populations of Tregs, CD4+EGFP+, and EGFP as well as CD8+EGFP+ and EGFP were added to the MLR in a 1:1 or 1:2 Tregs to CDP-670 labeled Tconv cells. Values are expressed as mean % ± SEM of suppression normalized to MLR proliferation in the absence of Tregs (0% inhibition of proliferation). n = 4. Student’s t test *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001

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