Fig. 5From: CD4+ and CD8+ regulatory T cell characterization in the rat using a unique transgenic Foxp3-EGFP modelRNAseq analyses of EGFP+ and EGFP− within CD4+ and CD8+ T cells. TCR+CD25highCD127lowCD4+ and TCR+CD45RClow/− EGFP+ and EGFP− T cells were cell sorted and RNAseq analyses were performed comparing CD4+EGFP+ vs. CD4+EGFP− cells (left column), and CD8+EGFP+ vs. CD8+EGFP− cells (middle column) and CD8+EGFP+ vs. CD4+EGFP+ cells right column. A Principal components analysis (PCA) of all samples and of expressed genes. B Volcano plots. (Left) Light green indicates overexpression in CD4+EGFP+ vs. CD4+EGFP− cells and black of CD4−EGFP+ vs. CD4+EGFP+ cells. (Middle) Dark green indicates overexpression in CD8+EGFP+ vs. CD8+EGFP− cells and black of CD8−EGFP+ vs. CD8+EGFP+ cells. (Right) Light green indicates overexpression in CD4+EGFP+ vs. CD8+EGFP+ cells and dark green of CD8−EGFP+ vs. CD4+EGFP+ cells. C Heatmaps of all differentially expressed genes. Low expression levels are in blue, mean expression levels are in white, and high expression levels are in red. Each sample number is depicted on the topBack to article page