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Fig. 5 | BMC Biology

Fig. 5

From: CD4+ and CD8+ regulatory T cell characterization in the rat using a unique transgenic Foxp3-EGFP model

Fig. 5

RNAseq analyses of EGFP+ and EGFP within CD4+ and CD8+ T cells. TCR+CD25highCD127lowCD4+ and TCR+CD45RClow/− EGFP+ and EGFP T cells were cell sorted and RNAseq analyses were performed comparing CD4+EGFP+ vs. CD4+EGFP cells (left column), and CD8+EGFP+ vs. CD8+EGFP cells (middle column) and CD8+EGFP+ vs. CD4+EGFP+ cells right column. A Principal components analysis (PCA) of all samples and of expressed genes. B Volcano plots. (Left) Light green indicates overexpression in CD4+EGFP+ vs. CD4+EGFP cells and black of CD4EGFP+ vs. CD4+EGFP+ cells. (Middle) Dark green indicates overexpression in CD8+EGFP+ vs. CD8+EGFP cells and black of CD8EGFP+ vs. CD8+EGFP+ cells. (Right) Light green indicates overexpression in CD4+EGFP+ vs. CD8+EGFP+ cells and dark green of CD8EGFP+ vs. CD4+EGFP+ cells. C Heatmaps of all differentially expressed genes. Low expression levels are in blue, mean expression levels are in white, and high expression levels are in red. Each sample number is depicted on the top

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