Fig. 6

Conversion of EGFP⁻ T cells in EGFP⁺ induced Treg. Spleen cells from Foxp3-EGFP animals were used to sort Tconv CD4⁺CD25⁻EGFP⁻ Treg and T CD8⁺CD45RC^low/−EGFP⁻ cells, labeled them with CPD-670 and cultured for 3 days in the presence of anti-CD3/CD28 mAbs, IL-2 and in the absence or presence of hTGFbeta. A Cells that proliferated (CPD-670low/neg) were then analyzed for EGFP expression. Left graphs show mean ± SEM of 3 different experiments and right histograms show one representative analysis. B Suppressive assays using EGFP⁺ (CD4⁺ or CD8⁺⁾ vs. EGFP⁻ cells (CD4⁺ or CD8⁺) from the same cultures at different ratios with CD4⁺ Tconv responder cells. n = 4, mean ± SEM. Student’s t test *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001