Fig. 5

A ThT fluorescence aggregation assay of the IgLC samples at pH 3 in the absence of glass beads measured in high-binding surface plates under agitation conditions. The different colour shades indicate the different monomer concentrations: light 5 μM; intermediate 35 μM; dark 100 μM. Overview of the effect of pH 3 on IgLC aggregation assayed by B increase of ThT fluorescence intensity, C the halftime of the aggregation and D relative fraction of aggregated protein determined by measuring the remaining soluble content after centrifuging the end product of the aggregation reactions. The three replicates per condition were combined before centrifugation