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Fig. 2 | BMC Biology

Fig. 2

From: Aedes aegypti exhibits a distinctive mode of late ovarian development

Fig. 2

PGC mass increases during the larval stage. A, A’, B, B’, C, C’, D, D’, E, E’ Confocal images of the germline during L1 (A), L2 (B), L3 (C), eL4 (D), and wL4 (E) stages with Vasa (green) and DNA (white) staining. A, A’, B, B’, C, C’, D, D’ PGCs continue to increase and form PGC mass from L1 to eL4. E, E’ PGC mass breaks into smaller clusters (one cluster indicated by a yellow rectangle) during the wL4 stage. F Quantification of PGC number from L1 to wL4 stages. Data are represented as mean ± SEM. The number of samples (n) in each group is shown above the X-axis. See Additional file 2 for the detail of counting information. Yellow dashed lines in B, B’, C, and C’ mark somatic cells on both ends. Scale bar in A is 50 µm

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