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Fig. 3 | BMC Biology

Fig. 3

From: Aedes aegypti exhibits a distinctive mode of late ovarian development

Fig. 3

PGC proliferates as interconnected cysts. Confocal images of the germline at 24 h AEL (A), 48 h AEL (B), 60 h AEL (C), L1 (D), L2 (E), L3 (F), and eL4 (G) with Vasa (green), α-Spec/phalloidin (red), DNA (blue), and PH3 (white) staining. A The gonad shows no PH3-positive PGC at 24 h AEL. BG PH3-positive PGCs (white) appear to form a cyst connected by fusome (red). H Quantification of PH3-positive cyst size from 24 h AEL to eL4 stages. Data are represented as mean ± SEM. I The percentage of PH3 + PGC cyst of a size that corresponds to a power of two. The number of samples (n) in each group is shown above the X-axis. The dash lines mark the PH3 + PGC cysts. Scale bar in A indicates 50 µm (for AF). Scale bar in G indicates 50 µm

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