Fig. 6
From: Aedes aegypti exhibits a distinctive mode of late ovarian development
Ecdysone signaling triggers the ovariole formation during metamorphosis. Confocal images (A–C) and zoomed for outlined areas of eL4 ovaries with mocked treatment (A, A’) and 20E treatment (B, B’, C, C’) with Vasa (green), α-Spec/phalloidin (red), and DNA (white) staining. A, A’ Mock-treated ovary shows wild-type structure. B, B’, C, C’ 20E-treated ovary exhibits precocious developmental features, including PGC mass elongation (weak phenotype, B, B’) and pre-ovariole formation (strong phenotype, C, C’). D Quantification of percentages of the ovaries with wild-type, weak, and strong phenotypes from three pooled independent replicates using the chi-squared test. The number of samples (n) in each group is shown above the X-axis. ***p < 0.001. Yellow line in C’ marks one TF stack. Scale bar indicates 50 µm