Table 1 Resource used in this study
From: Aedes aegypti exhibits a distinctive mode of late ovarian development
Reagent or resource | Source | Identifier |
|---|---|---|
Antibodies | ||
Guinea pig polyclonal anti-AaegVasa | This paper | N/A |
Rabbit polyclonal anti-cleaved Caspase3 (D175) | Cell Signaling Technology | Catalog # 9661: RRID: AB_2341188 |
Mouse monoclonal anti-histone H3 (phospho S10) | Abcam | Catalog # ab14955; RRID: AB_443110 |
Rabbit polyclonal anti-Dm-α-Spec | Liu et al., 2010[55] | N/A |
Alexa Fluor 488 AffiniPure goat anti-mouse IgG (H + L) | Jackson ImmunoResearch Laboratories | Catalog # 115–545-146; RRID: AB_2307324 |
Goat anti-guinea pig IgG (H + L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 | Invitrogen | Catalog # A-11073; RRID: AB_2534117 |
Cy3 AffiniPure goat anti-rabbit IgG (H + L) | Jackson ImmunoResearch LaboraInss | Catalog # 111–165-003; RRID: AB_2338000 |
Goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 405 | Invitrogen | Catalog # A-31553; RRID: AB_221604 |
DyLight 405 AffiniPure goat anti-rabbit IgG (H + L) | Jackson ImmunoResearch Laboratories | Catalog # 111–475-003; RRID: AB_2338035 |
Chemicals | ||
TO-PRO-3 iodide (642/661) | Invitrogen | Catalog # T3605 |
Hoechst 33,342 | Invitrogen | Catalog # H21492 |
Alexa Fluor 546 phalloidin | Invitrogen | Catalog # A22283 |
Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 555 dye | Invitrogen | Catalog # C10338 |
20-Hydroxyecdysone | Sigma-Aldrich | Catalog # H5142 |
Rapamycin | LC Laboratories | Catalog # R-5000 |
Experimental models: organisms/strains | ||
Aedes aegypti (NEA-EHI strain) | EHI, NEA | N/A |
Aedes aegypti (Liverpool strain) | Chun Hong Chen Lab, NHRI | N/A |
Culex quinquefasciatus (NEA-EHI strain) | EHI/NEA | N/A |
Anopheles sinensis (NEA-EHI strain) | EHI, NEA | N/A |
Software and algorithms | ||
GraphPad Prim 6 | GraphPad Software | RRID:SCR_002798 |
FIJI | Open source [56] | RRID:SCR_002285 |
Illustrator | Adobe | RRID:SCR_010279 |
Photoshop | Adobe | RRID:SCR_014199 |
Leica Application Suite X | Leica | RRID:SCR_013673 |
Oligonucleotides | ||
AaegVasa_EcoR1 F | GCGGATCCTTCGGCGGTGGCGACAATGA | |
AaegVasa_Xho1 R | CGCTCGAGTCAGGTCTGGGCACAGGCCATTA | |
Aaegα-Spec T7 F1 | TAATACGACTCACTATAGGGATGGAACAGTTTACCCCCAAGGAGG | |
Aaegα-Spec T7 R1 | TAATACGACTCACTATAGGGCTGTTGCAATTTCATTCCCTTTTCG | |
Aaegα-Spec T7 F2 | TAATACGACTCACTATAGGGGAACACCGTACCGAGGTAGATGCCCGC | |
Aaegα-Spec T7 R2 | TAATACGACTCACTATAGGGACGATCCAACACATCTGCACGCTTGTCG | |