Fig. 1

Acute cold exposure results in increased levels of triglycerides in skeletal muscle. A Experimental plan for short-term cold exposure. Muscle and adipose tissue were isolated from mice maintained at room temperature (RT, 23 °C) or cold (4 °C) for 3 days (n = 8). B–D Triglyceride (TG), cholesterol (CHO), and glucose levels in serum (n = 6). E TA muscle weight of the RT and COLD group mice at 10 weeks of age (n = 6). F Representative images of H&E staining of TA muscles of the RT and COLD group mice at 10 weeks of age. G Representative immunofluorescence staining of α-laminin in the TA muscle of the RT and COLD group mice at 10 weeks of age. H Quantitative PCR (qPCR) analysis of Myod1, Myog, Mymk, and Mymx in TA muscle during the cold challenge (n = 6). I TG content from TA muscle of the RT and COLD group mice at 10 weeks of age (n = 6). J–K Western blot of FABP4 protein levels and quantitative analysis in TA muscle during the cold challenge. L qPCR analysis of Cebpa, Adipoq, Fabp4, Pparg, and Plin1 in TA muscle during the cold challenge (n = 6). Error bars represent s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test