Fig. 5
From: Cold exposure alters lipid metabolism of skeletal muscle through HIF-1α-induced mitophagy
Cold exposure induced mitophagy and mitochondrial dysfunction. A Heatmap showing the differentially expressed genes related to mitophagy after cold exposure (n = 4). B Correlation analysis of mRNA expression of Adipoq with Bnip3 or Pink1 in TA muscle (n = 4). r, Pearson’s correlation coefficient. C Immunoblot analysis of mitochondrial-associated LC3B levels in mitochondrial-enriched fraction from the RT and COLD-treated mice, VDAC was used as a loading control. D Representative TEM images of TA muscle from the RT and COLD-treated mice (n = 4). E mtDNA levels in TA from the RT and COLD-treated mice (n = 6). F qPCR analysis of mitochondrial respiratory chain subunit genes in TA muscle. Mt-ND1, mitochondrially encoded NADH dehydrogenase 1; Mt-Co1, mitochondrial cytochrome c oxidase 2 gene; Mt-Co2, mitochondrial cytochrome c oxidase 2 gene (n = 4). G Western blot of CPT1α, CPT1β, and UCP2 protein expression in TA muscle from the RT and COLD-treated mice, GAPDH was used as a loading control. H qPCR analysis of genes related to mitochondrial biogenesis in TA from the RT and COLD-treated mice (n = 4). I qPCR analysis of genes related to fatty acid oxidation in TA from the RT and COLD-treated mice (n = 4). Error bars represent s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test