Fig. 6
From: Cold exposure alters lipid metabolism of skeletal muscle through HIF-1α-induced mitophagy
Activated HIF1a signaling regulates fat deposition and mitophagy in response to the cold challenge. A Heatmap showing the differentially expressed genes related to HIF-1α signaling (n = 4). B mRNA expression of Hif-1α and Hif-2α in TA from the RT and COLD-treated mice (n = 4). C Western blot of HIF-1α protein expression in TA from the RT and COLD-treated mice, GAPDH was used as a loading control. D Oil red O staining in primary myoblasts from normoxia (21%) and hypoxia (1%) for 48 h. E Oil red O and BODIPY staining from the control (CON) and COCL2-treated C2C12 myoblasts for 24h. F TG contents from CON and COCL2-treated C2C12 myoblasts for 24h (n = 6). G Immunoblot analysis of FABP4 levels from the CON and COCL2-treated C2C12 myoblasts for 24h. H qPCR analysis of genes related to lipid metabolism from the CON and COCL2-treated C2C12 myoblasts for 24 h (n = 4). I, J Immunoblot analysis of LC3B, CPT1B, and BNIP3 levels from the CON and COCL2-treated C2C12 myoblasts for 24 h. K qPCR analysis of genes related to autophagy and mitophagy from the CON and COCL2-treated C2C12 myoblasts for 24h (n = 4). L mtDNA levels in the CON and COCL2-treated C2C12 myoblasts for 24h (n = 6). Error bars represent s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test