Fig. 5
From: Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models
Determination of endogenous HTT allele ratios in genetically corrected HD iPSCs and non-HD iPSCs. a Percentage ratio of HTT WT allele obtained with ddPCR HTT_SNP7 assay (or HTT_SNP5 regarding C7522 cells) using gDNA from indicated iPSC lines. b,c Results from ddPCR are presented as a mean WT or corrMUT HTT transcript allele abundance, calculated based on results from all three HTT assays (HTT_SNP2, HTT_SNP5, and HTT_SNP7), in genetically corrected C39 (b) and C105 (c). d Ratio of WT alleles of HTT transcript (WT-1 and WT-2) in control C7522 cell line. Data were analyzed using unpaired t test. For all experiments presented in this figure n=3. Two-tailed p value < 0.05 was considered significant and is depicted in the figure by: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. All data are presented as means ± SD. Individual data values are available in Additional File 13