Fig. 6

Silencing of endogenous HTT expression in HD NSCs. a Endogenous HTT expression level after HD NSCs transfection with indicated oligonucleotides (siRL, A2, siHTT) assessed using ddPCR. b HTT WT and MUT allele abundance after transfection with indicated oligonucleotides. Results in a and b were normalized to HTT expression level in cells transfected with control siRNA targeting Renilla luciferase (siRL). Mean values from results obtained using HTT_SNP2, HTT_SNP5, and HTT_SNP7 are presented. Two-way ANOVA with Tukey’s multiple comparison test was used to analyze changes in HTT expression level [WT (*) or MUT (#) allele], while unpaired t test was used to determine significance between WT and MUT HTT allele downregulation for each oligonucleotide (†). For all experiments presented in this figure n=3. Two-tailed p value < 0.05 was considered significant and is depicted in the figure by: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. All data are presented as means ± SD. Individual data values are available in Additional File 13