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Fig. 7 | BMC Biology

Fig. 7

From: Allele-specific quantitation of ATXN3 and HTT transcripts in polyQ disease models

Fig. 7

Analyses on brain tissues of Hu128/21 mice aged 4 and 10 months. a Abundance of HTT transgene WT allele determined using HTT_SNP5 ddPCR assay on gDNA obtained from three Hu128/21 mice. b Copy number of HTT transgene in Hu128/21 mice, calculated using HTT_SNP7 assay, compared to the reference gene Rpp30. c,d HTT WT transcript abundance in midbrain (MB), olfactory bulb (OF), cortex (CTX), and striatum (STR) of 4-month-old (c) and 10-month-old (d) Hu128/21 mice. e,f Comparison of HTT WT transcript abundance in analyzed brain tissues between 4- and 10-month-old mice according to HTT_SNP2 assay (e), and according to HTT_SNP5 assay (f). Data were analyzed using unpaired t test (b), ordinary one-way ANOVA (c, d), or two-way ANOVA (e, f) with Sidak multiple comparison test. In e, we present results obtained by two-way ANOVA analysis, where all 4 tissues were grouped together and analyzed by the significance of the time point factor. For all experiments presented in this figure n=3. Two-tailed p value < 0.05 was considered significant and is depicted in the figure by: *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. All data are presented as means ± SD. Individual data values are available in Additional File 13

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