Fig. 2

Study of canine mammary tumoroids drift. A Diagram showing the strategy used to generate the different types of tumoroids. After tumor digestion, a part of the cells were frozen and then thawed to generate the “Frozen Cell Tumoroids.” The remaining cells were used to generate the “Fresh tumoroids.” A part of these tumoroids were cryopreserved and then thawed, corresponding to the “Frozen Tumoroids.” The three types of tumoroids were compared at the same time point post-culture at date 1 (4–5 weeks) or date 2 (6–7 weeks). B Evaluation of the efficiency of tumoroids formation for the 6 cases in triplicates (n = 6). The average of the triplicates is shown, and error bars mean SD. C Representative images of the three types of canine tumoroids at passage 1, 2, and 3 after date 1. Scale bar (200 μm) is indicated. D H&E staining comparison of fresh, frozen, and frozen cells tumoroids. Scale bar = 100 μm (n = 3)