Fig. 7

Proteomics analysis comparing the primary tumor to their derived tumoroids. A Venn diagram representing specific proteins in tumor of origin, Fresh, Frozen, and FrozenCell tumoroids (n = 3 for each condition). B Biological processes of the specific proteins identified in primary tumors using FunRich and ClueGO (n = 3 for each condition). C Hierarchical clustering of the most variable proteins between the tumor of origin and the 3 tumoroid conditions (n = 3 for each condition, ANOVA with permutation-based FDR < 0.05). Biological processes distribution of underexpressed (D) and overexpressed (E) proteins in tumors compared to tumoroids using FunRich and ClueGO (n = 3 for each condition)