Fig. 8

Drug response of canine and human tumoroids to paclitaxel. A Representative bright field images showing the morphology of the three types of tumoroids after 7 days treatment with paclitaxel at different concentrations. Scale bar (100 μm) is indicated. B Quantification of the tumoroids viability following paclitaxel treatment. Tumoroids were generated from three different canine mammary tumors (TM-04, TM-05, and TM-06), and drug response was compared between fresh, frozen, and frozen cell tumoroids. Data are the means ± SD (n = 3 for each condition). C Tumoroids were generated from human and canine luminal and triple-negative mammary tumors, and drug response was compared between human and canine tumoroids. Different concentrations of drug were used and compared to non-treated tumoroids. Data are the means ± SD (n = 3 for each condition)