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Fig. 2 | BMC Biology

Fig. 2

From: The chromosome-level genome and key genes associated with mud-dwelling behavior and adaptations of hypoxia and noxious environments in loach (Misgurnus anguillicaudatus)

Fig. 2

Alterations in fos (ID: Mis0086400.1) associated with the mud-dwelling ability of loach Misgurnus anguillicaudatus. a Alignment of the protein sequences of fos from the five species: M. anguillicaudatus, Danio rerio, Mastacembelus armatus, Ophiocephalus argus, and Triplophysa tibetana. The gray bar with blue stripes shows the exon-intron structure of fos and the red word (Q) with salmon color back ground indicates the specific mutation in the fos gene of the loach. b Whole-mount in situ hybridization (WISH) analysis of fos. Red dotted areas indicate the expression signals. hpf, hours post-fertilization. c Micro-computed tomography (Micro-CT) analysis of wild-type loach (WT), fos-deletion loach (fos−/−), and Cobitis sinensis. d–f The parameters of spine bones: BMD, BV/TV and Tb.Th. BMD, bone mineral density; BV/TV, bone volume over total volume; Tb.Th, trabecular thickness. g Trap staining and toluidine blue staining of spines from WT and fos−/− loach. Blue arrows indicate the osteoclasts; the salmon color arrows indicate the chondrocytes. fos, Proto-oncogene c-Fos. Different letters above error bars indicate significant difference (p < 0.05). *** extremely significant difference (p < 0.001)

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BMC Biology

ISSN: 1741-7007

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