Fig. 9
From: Integrative single-cell RNA-seq and ATAC-seq analysis of myogenic differentiation in pig
Cell–cell communication analysis in the developing pig somite. A, B Heatmaps showing the number of cell–cell interactions in the scRNA-seq dataset of myogenic cells (A) and all somite cells (B), as inferred by CellPhoneDB. Dark blue and dark red colors denote low and high numbers of cell–cell interactions, respectively. C CellPhoneDB-derived measures of cell–cell interaction scores and p values among myogenic cells. Each row shows a ligand–receptor pair, and each column shows the two interacting cell types binned by cell type. Columns are sub-ordered by first interacting cell type into Pax3+ progenitors, myogenic progenitors, myoblasts, and myocytes. The color scale denotes the mean values for all the interacting partners, where the mean value refers to the total mean of the individual partner average expression values in the interacting cell-type pairs. D CellPhoneDB-derived measures of cell–cell interaction scores and p values between myogenic cells and non-myogenic cells. Columns are sub-ordered by interacting myogenic cell type into Pax3+ progenitors, myogenic progenitors, myoblasts, and myocytes. E A diagram demonstrating the regulation of pig skeletal muscle ontogeny during embryonic development. In this model, during E16 to E 28, chromatin accessibility regulates the expression of classical and newly identified myogenic-related genes, which in turn promotes myogenic lineage fate determination and further myogenic differentiation