Fig. 6
From: Formation and characterization of BMP2/GDF5 and BMP4/GDF5 heterodimers
Comparison of inhibitory activity of protein antagonists for heterodimers and homodimers. Inhibition of BMP signaling induced by BMP2 (light blue), BMP4 (dark blue), GDF5 (green), BMP2/GDF5 (light purple), and BMP4/GDF5 (dark purple) by Gremlin 2 (A), Noggin (B), and NBL1 (C), as measured by luciferase reporter assay in BRITER osteoblast cells. Representative experiment shown. Data normalized to untreated control and analyzed using GraphPad Prism using non-linear regression with variable slope and least squares fit. Average IC50 of N=3 experiments with 95% confidence interval