Fig. 3

Major proteins in isolated membrane ghosts and in supernatants after centrifugation of membrane ghosts of normal and sickle erythrocytes. The samples were separated by SDS-PAGE, transferred to nitrocellulose membrane, and stained with ponceau S (A). Lane 1: protein marker; lanes 2–4: isolated membrane ghosts of the normal erythrocytes from three healthy subjects; lanes 5-7: isolated membrane ghosts of the sickle erythrocytes from three sickle cell anemia patients; lanes 8–12: the supernatants after 1^st, 4^th, and 7^th centrifugation of membrane ghosts of normal erythrocytes from two healthy subjects; lanes 13–18: the supernatants after 1^st, 4^th, and 7^th centrifugation of membrane ghosts of sickle erythrocytes from two patients. B Quantitative analysis of the hemoglobin bands indicated by the white boxes in A (***P < 0.001)