Fig. 2.

Confocal microscopy imaging of the subcellular localization of exogenous cFPGS mRNA harboring its native 3′UTR. HeLa cells co-transfected with FLAG-cFPGS-3′UTR-24xMS2 (F-MS2) and MCP-GFP were grown in full growth medium (A, D), serum-free medium (16 h, B, E), and serum-repleted medium (1.5 h, C, F). Fixed cells were stained with Hoechst 33342 (nuclei, blue fluorescence, 405 nm) and phalloidin (white, 630 nm) and were scanned using a confocal microscope (×63 magnitude). F-MS2 mRNA was detected by the green fluorescence of bound MCP-GFP (488 nm). Arrows point to cell protrusions with accumulated mRNA. Scale bars denote 10 μm. Shown are representative images of three independent experiments