Fig. 3.From: Folylpolyglutamate synthetase mRNA G-quadruplexes regulate its cell protrusion localization and enhance a cancer cell invasive phenotype upon folate repletionDisruption of the GQ elements at the 3′UTR of cFPGS mRNA abolishes its transport to cell protrusions. HeLa cells were co-transfected with MCP-GFP and FLAG-cFPGS-3′UTR-24xMS2 (F-MS2) harboring either the WT UTR (A–E), a mutated GQ at position 116 (mGQ116, F–J), or a mutated GQ at position 377 (mGQ377, K–O). Following 16 h of serum deprivation, cells were supplemented with complete growth medium for 1.5 h, fixed and subjected to IF microscopy. The localization of F-MS2 mRNA is indicated by MCP-GFP (green fluorescence, 488 nm), FLAG-cFPGS protein was detected by an anti-FLAG antibody (magenta, 405 nm), and F-actin was stained by DyLight 650 Phalloidin (white, 630 nm). Cells were scanned using a confocal microscope (×63 magnitude). The scale bars denote 10 μm. Shown are representative images of three independent experimentsBack to article page