Fig. 4.

Time-lapse depiction of FA-induced cFPGS mRNA accumulation in a cell protrusion. HeLa cells deprived of FA for 13 days were co-transfected with MCP-GFP along with either F-MS2 (A) or F-MS2-dmGQ (B). The next day, the distribution of cFPGS mRNA (MCP-GFP, 488 nm) was monitored in single cells by live fluorescence imaging before (t = 0) and after the addition of 2μM FA to the growth medium. Cells were scanned with a confocal microscope (×63 magnitude). The scale bars denote 10 μm