Fig. 6.

cFPGS mRNA transport to cell protrusions is dependent on actin filaments. HeLa cells, deprived of FA for 12 days, were co-transfected with MCP-GFP along with either F-MS2 (A–T) or F-MS2-dmGQ (U–Y). The next day, each cell group was split into different wells and left to grow in FA-free medium for an additional 24 h. Cells were pre-treated for 1 h with 0.1% DMSO (vehicle, A–E), 40 μM VBT (F–J), 10 μM NCZ (K–O), or 250 nM LAN B (P–Y) and supplemented with 2 μM FA for 15 min before fixation and IF microscopy. The localization of cFPGS mRNA is indicated by MCP-GFP (green fluorescence, 488 nm), FLAG-cFPGS protein was detected by an anti-FLAG antibody (red, 405 nm), and F-actin was stained by DyLight 650 Phalloidin (white, 630 nm). T The silhouette of the cells was outlined with a purple line. Cells were scanned using a confocal microscope (×63 magnitude). The scale bars denote 10 μm. Shown are representative images of three independent experiments