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Fig. 8. | BMC Biology

Fig. 8.

From: Folylpolyglutamate synthetase mRNA G-quadruplexes regulate its cell protrusion localization and enhance a cancer cell invasive phenotype upon folate repletion

Fig. 8.

The 3′UTR of FPGS enhances cell migration under confined conditions. FA-deprived HeLa cells, co-transfected with either F-cFPGS (3′UTR-null) and BFP (A–C), F-MS2 and GFP (D–F, J, K), or F-MS2-dmGQ and RFP (G–I), were overlaid with FA-containing collagen and medium (A, D, G), FA-free collagen and medium (B, E, H), or FA-free collagen and FA-containing medium (C, F, I–K) as detailed in Figure S1. Following 40 h of incubation, cells were visualized by confocal microscopy using focus stacking (i.e., Z-stacks), and 3D semi-translucent images were generated. Each image is shown from both a bottom and top view, with a color gradient representing the depth within the image. J, K Images of the same cell with a 2.5-h interval, during which a string of vesicles detached from the cell protrusion, and a new bleb formed at the tip of the protrusion. Arrows point at vesicles released from cell protrusions. The scale bars denote 10 μm. Transition through the Z-stacks can be seen in the supplementary movies S1-S9

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