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Fig. 6 | BMC Biology

Fig. 6

From: Evolution of pathogenicity-associated genes in Rhizoctonia solani AG1-IA by genome duplication and transposon-mediated gene function alterations

Fig. 6

Silencing of Rs_01468 (LPMO) gene under diversifying selection compromises the pathogenesis of R. solani. A qRT-PCR-based expression analysis of Rs_01468 during the pathogenesis of R. solani in rice at different time points, compared to 0 dpi. The expression was normalized using R. solani 18S rRNA. B Disease symptoms, C disease index (% RVSC) and D pathogen load in rice tillers infected with Rs_01468 silenced R. solani compared to control (Rs_GT34 silenced and buffer treated), at 3 dpi. E Disease symptoms, F disease index and G pathogen load in tomato leaves infected with Rs_01468-silenced R. solani compared to control (Rs_GT34 silenced and buffer treated), at 3 dpi. The pathogen load (2−ΔCt) was estimated as an abundance of 18S rRNA of R. solani, upon normalization with rice 18S or tomato actin gene. The graph shows the mean values ± standard error of three biological replicates. “*” indicates a significant difference at p ≤ 0.01. LPMO, lytic polysaccharide monooxygenase; GT34, glycosyl transferase family 34 protein. Scale bar: 1 cm

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