Fig. 7

Rs_11537 (GNAT), a gene under purifying selection is important for the pathogenesis of R. solani. A qRT-PCR-based expression analysis of Rs_11537 and Rs_05222 genes during R. solani pathogenesis in rice at different time points, as compared to 0 dpi using rice actin gene for normalization. B Disease symptoms, C disease index (%RVSC) and D pathogen load in rice tillers infected with gene silenced (dsRNA treated) or buffer treated R. solani, at 3 dpi. E Disease symptoms, F disease index and G pathogen load in tomato leaves infected with gene-silenced (dsRNA-treated) or buffer-treated R. solani, at 3 dpi. The pathogen load (2^−ΔCt) was estimated as an abundance of 18S rRNA of R. solani, upon normalization with rice 18S or tomato actin gene. The graph shows the mean values ± standard error of three biological replicates. Scale bar: 1 cm