Fig. 2
From: Platelet P2Y1 receptor exhibits constitutive G protein signaling and β-arrestin 2 recruitment
P2Y1-R exhibits constitutive signaling in resting mouse and human platelets. a, b Washed murine (a) or human (b) platelets were incubated in the absence (basal, dashed line) or in the presence of ADP (100 μM), MRS2179 (10 μM), vehicle (DMSO), or the Gq inhibitor (100 nM) for 2 h and IP1 accumulation was quantified. Data represent the mean ± s.d. of 13 mice (a) or 16 healthy donors (b) and are expressed as the percentage of matched basal values. The statistical comparison between untreated (basal or vehicle) and treated (ADP, MRS2179 or Gq inhibitor respectively) platelets was assessed using Kruskal-Wallis test followed by Dunn’s post-tests (*p < 0.05; **p < 0.01; ***p < 0.001)