Fig. 7

Expression profiles of DMR-mapped genes during liver aging and/or regeneration. A Expression of aging DMR-mapped c-Myc, Rai1, GSK3β, and Nceh1 in 2, 8, and 16-m/o livers by qRT-PCR, indicated by light grey, dark grey, and black bars, respectively. B Expression of regenerative DMR-mapped Cbx6, Sox9, and Errf1 in 2-m/o livers after PHx. C Expression profiles of two genes mapped by age-inverse regenerative DMRs (C1, C2) and two DEGs mapped by early-late aging inverse DMRs (C3, C4). D1 Regeneration of 2-m/o and 16-m/o livers, measured by the ratio of dissected liver mass (for 0d) or remaining liver mass (for 1d, 2d, and 4d) to body weight. (D2) Regeneration of 2-m/o and 16-m/o livers, measured by the numbers of Ki67 + cells per high-power field (HPF = 0.126 mm.²). E Expression profiles of Sox9, Rap2c, Thrsp, Rspry1, and Kif4 in response to PHx at 16 m/o. Expression levels were normalized against that of Rplp0 as an internal reference and compared to their respective baseline (0d) or 2-m/o baseline (for E5) samples. Bars represent means (± s.e.m.) of six biological replicates with two technical repeats each (n = 12) in reference to the Rplp0 level of the same sample. *p < 0.05; **p < 0.01; ***p < 0.001