Fig. 2
From: Polycomb group genes are required for neuronal pruning in Drosophila
The components of PRC1 complex are required for dendrite pruning in ddaC neurons. A–H Live confocal images of ddaC neuron labelled by mCD8GFP at WP and 16 h APF. Somas of ddaC are marked by red arrowheads. Dendrites of control ddaC neurons were pruned away at 16 h APF (A, E), whereas ph RNAi-expressing ddaC neurons (B), ph505 ddaC MARCM clones (C), RU486-induced ph RNAi ddaC neurons (F), Pc15 ddaC MARCM clones (G) and Psc-Su(z)21.b8 MARCM clones (H) exhibited the dendrite pruning defects. Overexpression of ph-p partially rescued the pruning defects in ph505 ddaC MARCM clones (D). I Quantification of severing and fragmentation defects of ddaC neurons at 16 h APF. J Quantification of length of unpruned ddaC dendrites at 16 h APF. The number of neurons (n) in each group is shown on the bars. Error bars in all experiments represent ± SEM. Two-tailed Student’s t test was used to determine statistical significance for pairwise comparison, whereas one-way ANOVA with Bonferroni test was applied to determine significance for multiple-group comparison. **p < 0.01, ***p < 0.001. Three independent replicates were conducted. Scale bar represents 50 µm