Fig. 3From: Polycomb group genes are required for neuronal pruning in DrosophilaThe components of PRC2 complex are important for dendrite pruning in ddaC neurons. A–F, I,J Live confocal images of ddaC neuron labelled by mCD8GFP at WP and 16 h APF. Somas of ddaC are marked by red arrowheads. Dendrites of control ddaC neurons were pruned away at 16 h APF (A), whereas E(z)73 ddaC MARCM clones (B), E(z)731 ddaC MARCM clones (C), Su(z)122 ddaC MARCM clones (D), Su(z)124 ddaC MARCM clones (E) and esc21/Df trans-heterozygous mutant ddaC neurons (F) showed mild pruning defects. Compared to Pc15 MARCM ddaC neurons (I), Pc15, E(z)731 double mutant MARCM clones (J) showed the enhanced pruning defects. G, K Quantification of severing and fragmentation defects of ddaC neurons at 16 h APF. H, L Quantification of length of unpruned ddaC dendrites at 16 h APF. The number of neurons (n) in each group is shown on the bars. Error bars in all experiments represent ± SEM. Two-tailed Student’s t test was used to determine statistical significance for pairwise comparison, whereas one-way ANOVA with Bonferroni test was applied to determine significance for multiple-group comparison. n.s., not significant, **p < 0.01, ***p < 0.001. Three independent replicates were conducted. Scale bar represents 50 µmBack to article page