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Fig. 3 | BMC Biology

Fig. 3

From: Polycomb group genes are required for neuronal pruning in Drosophila

Fig. 3

The components of PRC2 complex are important for dendrite pruning in ddaC neurons. A–F, I,J Live confocal images of ddaC neuron labelled by mCD8GFP at WP and 16 h APF. Somas of ddaC are marked by red arrowheads. Dendrites of control ddaC neurons were pruned away at 16 h APF (A), whereas E(z)73 ddaC MARCM clones (B), E(z)731 ddaC MARCM clones (C), Su(z)122 ddaC MARCM clones (D), Su(z)124 ddaC MARCM clones (E) and esc21/Df trans-heterozygous mutant ddaC neurons (F) showed mild pruning defects. Compared to Pc15 MARCM ddaC neurons (I), Pc15, E(z)731 double mutant MARCM clones (J) showed the enhanced pruning defects. G, K Quantification of severing and fragmentation defects of ddaC neurons at 16 h APF. H, L Quantification of length of unpruned ddaC dendrites at 16 h APF. The number of neurons (n) in each group is shown on the bars. Error bars in all experiments represent ± SEM. Two-tailed Student’s t test was used to determine statistical significance for pairwise comparison, whereas one-way ANOVA with Bonferroni test was applied to determine significance for multiple-group comparison. n.s., not significant, **p < 0.01, ***p < 0.001. Three independent replicates were conducted. Scale bar represents 50 µm

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