Fig. 4
From: Polycomb group genes are required for neuronal pruning in Drosophila
PRC1 represses Abd-B and Scr expression in ddaC neurons. A Confocal images of ddaC neurons (green) immuno-stained with anti-Ubx, anti-Abd-A, anti-Abd-B or anti-Scr (magenta). ddaC somas are marked by dashed lines. ph RNAi ddaC neurons showed de-repression of Abd-A, Abd-B and Scr, but not Ubx. B Quantification of Hox protein expression levels in the nuclei of ddaC neurons expressing control and ph RNAi. C MARCM ddaC clones (green) derived from ph505, Pc15 and Psc-Su(z)21b.8 mutant alleles immuno-stained with anti-Abd-B (magenta). Non-clonal heterozygous ddaC controls were taken from the contralateral segment of their respective clones. ddaC somas are marked by dashed lines. D Quantification of Abd-B protein expression levels in the ddaC nuclei. The number of neurons (n) in each group is shown on the plots. Error bars in all experiments represent ± SEM. Two-tailed Student’s t test was used to determine statistical significance for pairwise comparison. n.s., not significant, **p < 0.01 ***p < 0.001. Three independent replicates were conducted. Scale bar represents 10 µm