Fig. 6

Ph knockdown and Abd-B overexpression led to downregulation of Mical expression. A–D Confocal images of ddaC neurons (green) at WP stage immuno-stained with anti-EcR-B1, anti-Sox14 or anti-Mical (magenta). ddaC somas are marked by dashed lines. ddaE somas are marked with asterisks. EcR-B1 and Sox14 expression levels were not reduced in ph RNAi (B), Abd-A-overexpressing (C) and Abd-B-overexpressing ddaC neurons (D). Mical expression levels were unaffected in Abd-A-overexpressing ddaC neurons (C) but significantly reduced in ph RNAi (B) and Abd-B-overexpressing ddaC neurons (D). E–G Quantification of EcR-B1, Sox14 and Mical expression levels. The number of neurons (n) in each group is shown on the plots. Error bars in all experiments represent ± SEM. Two-tailed Student’s t test was used to determine statistical significance for pairwise comparison, whereas one-way ANOVA with Bonferroni test was applied to determine significance for multiple-group comparison. n.s., not significant, *p < 0.05, ***p < 0.001. Three independent replicates were conducted. Scale bars represent 10 µm