Fig. 3
From: Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA
Indel frequency influences log fold change (LFC) viability scores. A–D Correlation between moving median of LFC in the frequency of sgRNAs targeting RPL8 or RPL15 and A indel frequency (day 21), B predicted off-target rank percentage, C percentage of gRNA-targeting cut sites within target gene coding sequences, and D percentage of predicted in-frame mutations. Gray dots indicate values for each individual sgRNA while black bold dots indicate the moving median of the 20 nearest neighbors. R indicates Pearson correlation coefficient r and p value is calculated by the two-tailed test. E, F Comparison of sgRNA fold changes between sgRNAs targeting near exon–intron junction (Junction) and those targeting within exon (Other)